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Histamine, octyl, and R- and S-octan-2-yl isocyanates were purchased from Sigma-Aldrich Co. PC (chicken egg), PS (porcine brain), and Chl were purchased from Avanti Polar Lipids. Trizma hydrochloride, Trizma base, sodium phosphate monobasic, sodium phosphate dibasic, NaCl, Hepes, calcein, EDTA, Triton X-100, SDS, D2O, ethanol, and chloroform were purchased from Sigma-Aldrich Co. Compressed N2, He, and O2 were supplied by Airgas. All buffers were prepared fresh and filtered through a 0.22-μm polyethersulfone membrane (Express PLUS, Millipore). For the SFG experiments, PBS (phosphate-buffered saline) and SDS were purchased from Sigma-Aldrich Co. Sodium chloride was purchased from Mallinckrodt Chemicals. Ultrapure water (Millipore MilliQ, 18.2 megohms·cm, ≤ 5 parts per billion total organic carbon) was used for all solutions. Equilateral CaF2 prisms were obtained from Crystran Ltd.
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