2018 AAU Junior Olympic Games – Entries | Acid Black 24

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Abstract: A condensation trap comprising an inlet chamber, a vent chamber and an outlet chamber. The inlet chamber is configured to receive condensate fluid through an external opening therein. The vent chamber is in fluid communication with the inlet chamber via a first passageway that includes an internal opening of the inlet chamber. The internal opening is located substantially at an opposite end of the vent chamber as the external opening. The outlet chamber is in fluid communication with the vent chamber via a second passageway that includes an internal opening in a sidewall of the vent chamber and an interior opening in an end of the outlet chamber. The outlet chamber is configured to transmit the condensate fluid through an exterior opening located at an opposite end of the outlet chamber. A vent volume portion is greater than a total volume of an internal space of the inlet chamber.

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Pallbearers included grandsons: Adam Scott, Shane Scott and Ethan Staples and friends Lamar “Turkey” Cantrell, Larry Garrison and Joe Harris. Steve “Red” Osborne will serve as an honorary pallbearer.

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The HCC38 cells were cultured in a flask in RPMI 1640 medium (Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (ScienCell) in an incubator (5% CO2 at 37°C). Glucose (4.5 g liter−1), 5 mM MgCl2, transfer RNA (0.1 mg ml−1), and bovine serum albumin (1 mg ml−1) were dissolved in PBS with CaCl2 and MgCl2 to obtain a homogeneous solution (binding buffer). After that, the cells at the logarithmic growth phase were centrifuged at 800 rpm for 3 min to separate them from the medium and then dispersed in the binding buffer to form a cell suspension. Cell numbers were obtained by a cell counter (Nsum). About 100 μl of cell suspension was dropped onto the aptamer-modified silicon probe and incubated at 37°C for 60 min. The cells could be captured by the specific binding between the aptamer and HCC38 cells. The captured cells on silicon foils (N) were calculated by counting the number of cells after capture (Nres) and calculating using the equation N = Nsum − Nres.


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