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Abstract: A hole is formed through the two or more parts, and a hollow tube is extended through the hole. A first plug is inserted into a first end of the tube, and a second end of the tube is pulled through the hole until the first plug is urged against the two or more parts. The second end of the tube is then cut so that the second end is flush with the two or more parts. A second plug is then inserted into the second end of the tub.

Abstract: In a general aspect, a subterranean region is simulated using a finite element mesh and a boundary element mesh. In some aspects, a finite element mesh is generated over a domain representing a subterranean region. The finite element mesh defines elements that represent respective sub-regions of the subterranean region. The elements include a first element having a boundary defined by the finite element mesh, and at least a portion of the boundary of the first element represents a discontinuity in the subterranean region. A boundary element mesh is generated over the boundary of the first element, and the subterranean region is simulated using the finite element mesh and the boundary element mesh.

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Note that water molecules form a more compact water wire motif in R-HC8 and S-HC8 compared with HC8. The I-quartet water channels of rhomboidal shape (20.1 × 22.9 Å2 for HC8 and 22.4 × 14.9 Å2 for R-HC8 and S-HC8, considering a projection in a plane and not taking into account the van der Waals radii of terminal sites) determine a pore gap in the 2.6 Å channel, very close to the narrowest constriction observed in AQP water channels (2.8 Å) perfectly accommodating water wires’ binding and conduction (22, 23). Moreover, these structures confirm that precise interactions constrain the orientation of water molecules that move collectively while maintaining H-bond interactions within the I-quartet pore. A similar occupancy by partially hydrated cations would imply a change in the energy difference between H-bonding configurations of hydrated cations in the I-quartet pore. The binding behavior of I-quartets is optimal for maximizing the pure water translocation rates (24). From the x-ray single-crystal data of the I-quartets reported here, it can be concluded that (i) the water structure in individual channels of both chiral R-HC8 and S-HC8 or achiral HC8 imidazoles form oriented water wires. Such a directional chiral ordering of confined water is imposed by relative positioning of the imidazole dimers within the channel. (ii) For HC8, because of the symmetry inversion centers, two vicinal water–filled channels are oppositely oriented, so a racemic mixture of water wires of both chiralities are present, whereas for the chiral nonsymmetric R-HC8 and S-HC8 channels, water wires present a unique dipolar orientation of all successive channels (Fig. 1, F and G).

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