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The HCC38 cells were cultured in a flask in RPMI 1640 medium (Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (ScienCell) in an incubator (5% CO2 at 37°C). Glucose (4.5 g liter−1), 5 mM MgCl2, transfer RNA (0.1 mg ml−1), and bovine serum albumin (1 mg ml−1) were dissolved in PBS with CaCl2 and MgCl2 to obtain a homogeneous solution (binding buffer). After that, the cells at the logarithmic growth phase were centrifuged at 800 rpm for 3 min to separate them from the medium and then dispersed in the binding buffer to form a cell suspension. Cell numbers were obtained by a cell counter (Nsum). About 100 μl of cell suspension was dropped onto the aptamer-modified silicon probe and incubated at 37°C for 60 min. The cells could be captured by the specific binding between the aptamer and HCC38 cells. The captured cells on silicon foils (N) were calculated by counting the number of cells after capture (Nres) and calculating using the equation N = Nsum − Nres.
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“In March of 2012, when examining the crystallization of aspirin from the melt, New York University’s Professor Alexander Shtukenberg discovered the concomitant crystallization of banded spherulites (concentric optical rings) of form I and smooth spherulites of an unknown form.”
”The key beneficiary of tourism is generally central government and the key cost bearer of providing facilities for tourism tends to be local government – this mismatch of cost and benefit is the core issue for tourism.”
Gujarat is also a major source of dyes used for coloring textiles and plastic household goods. The fall in the rupee has supplied Indian exporters a competitive advantage, reducing the price of their wares in dollar terms.
It took years of setbacks and several collaborations before Dr. Hu and colleagues achieved high yields of aspirin IV and performed its structural analysis.
The x-ray structure of HC8 reveals that two conformers of HC8 are present in the solid state: one elongated stretching to their maximum theoretical length, 19.8 Å, and one contracted at 19.1 Å presenting two gauche conformations (Fig. 1, A to C, and fig. S1). The crystal packing of chiral R-HC8 (Fig. 1B) or S-HC8 (Fig. 1C) reveals compact bent conformations with the heptyl chains orthogonally disposed to the urea moieties. The homomonomeric H-bonding association of the urea-urea planar ribbons of the neighboring ureas lie in the same plane with respect to one another, as previously observed (15). The NH—O=C– H-bond (dO—H of 1.90 Å) distances are the same along the ribbon and are consistent with other urea systems (15–18). Accordingly, continuous asymmetric layers of HC8 and R-HC8 or S-HC8 are generated in the solid state, such that the imidazole moieties are positioned at one end of each ribbon and strong hydrophobic van der Waals interactions between the alkyl chains stabilize the ribbon-like superstructures on the opposite side.
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