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We further investigated the PD-L1 expression on TNBC surfaces using Raman mapping. We collected a topographic image of 50 μm by 50 μm from the integrated intensity of the peak at 1530 cm−1. The first column of Fig. 5L shows the Raman mapping images of the PD-L1 expression level of three types of TNBCs. The color-false images revealed that the expression level of PD-L1 on three types of TNBCs was MDA-MB-231 > HCC38 >> MCF-7. Both the calculated results and Raman mapping images were consistent with those of the commercial enzyme-linked immunosorbent assay (ELISA) kit (fig. S7B). The Raman imaging technique mentioned above was further successfully used to monitor dynamic PD-L1 expression on three different TNBCs in response to IFN-γ, which was a type of biomolecule reported to upgrade the expression of PD-L1 on cell surfaces (38). Three types of IFN-γ–treated TNBCs progressively increased with the concentrations of IFN-γ (Fig. 5, M to P). Besides concentration-dependent mapping, to provide additional evidence that our probe selectively targets PD-L1 on cell surfaces with high sensitivity, we further conducted time-dependent Raman mapping experiments for IFN stimulation. Figure S8 shows the time-dependent responses for IFN-γ during the course of the experiment. The Raman peak obtained from IFN-γ–treated cells increased as the IFN-γ incubation time increased and reached a plateau after 48 hours. Both the histogram and Raman mapping images indicated that IFN-γ treatment significantly stimulated the expression of PD-L1 on cell surfaces within 48 hours. All these results confirmed the high sensitivity and selectivity of the MGT-Abs-CuPc probe for Raman bioanalysis and imaging.

She says the FDA has concluded that lead acetate is no longer safe.  Multiple brands have already done the right thing and removed this from their product.  Remember:  Brands have a year from the date of the rule to remove a harmful ingredient from their items.

Nestlé is currently the fourth largest chocolate confectioner in Australia with Mondelēz, Mars and Lindt & Sprüngli the top players in the category and Ferrero in fifth, according to Euromonitor data.

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Claudine Schneider had been elected to the U.S. House of Representatives in 1980 in the 2nd Congressional District.  Susan Farmer won the Secretary of State post two years later in 1982. Violet was the first female Attorney General in the United States when she was elected by Rhode Island voters in 1984. The new decade had ushered in a new era in Rhode Island politics. All three were Republicans.

Dr. Caudle says lead acetate has been used in “progressive” hair dyes.  These are the ones that change the color over time.

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