Serbia, Brazil, Dominican Republic and Puerto Rico will now proceed to the next round. Leonida Kasaya and Sharon Chepchumba scored five points each for Kenya while Lisvel Mejia and Brayelin Martinez added 12 points each in the victory for the Dominicans.

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In this work, a novel ternary, plasmon-free MGT SERS probe platform has been first successfully developed for the sensitive and selective detection of various types of TNBCs and for the evaluation of PD-L1 expression at the single-cell level. First of all, a ternary nanostructure has been designed and synthesized layer by layer and used as a plasmon-free SERS substrate. The EF value of MGT for CuPc has been estimated to be 8.08 × 106, which is one order of magnitude higher than the highest value reported by far. Both experimental results and theoretical DFT calculation have revealed that introduction of TiO2 to the MG nanocomposites induces a significant enhancement of the Raman intensity of individual molecules with high selectivity, which should be attributed to the resonance Raman effect of CuPc, CT between GO and TiO2 in MGT nanocomposites, and the enrichment of porous TiO2. The developed SERS substrate has been designed as a nanoprobe providing remarkable properties for quantification of PD-L1 expression on TNBC surfaces with a detection limit down to ~3 cells. Besides, this SERS probe has been used to measure in situ the expression of PD-L1 on TNBC surfaces at the single-cell level and to monitor the expression variation and distribution of PD-L1 during drug treatment through Raman mapping images. Thus, a probe with ultrahigh EF based on CM has been provided, which can also act as a robust and rapid cytosensor for cancer cells and membrane protein with selectivity, sensitivity, and accuracy. In addition, MGT nanocomposites have demonstrated excellent recyclability under visible light. This versatile SERS cytosensing platform has been a promising tool for detection of PD-L1 expression with great clinical value for diagnosis and treatment of human TNBCs, which can be expanded for detection of other trace cancer biomarkers and for reliable diagnosis of cancer and other diseases.

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Abstract: A low-debris low-interference semi-solid well perforator having selectively variable free volume and method for providing such is disclosed according to one or more embodiments. The perforator may include a charge tube holding an independently floating axial stack of selectively variable divider segments, each having one or more concavities formed in upper and lower sides. The segments are arranged so that concavities of adjacent segments form sockets, into which shaped charges are located. The segments provide support to minimize deformation of shaped charge cases yet provide less than 360 degrees circumferential contact about the shaped charges to form selectively variable voids for collecting debris and spall resulting from detonation. The voids and floating segments attenuate detonation shock interference. A debris guard prevents debris from entering the wellbore. Relieving slots in the debris guard attenuates transmission of shock interference through the debris guard.

Inventor(s): Keith E. Matheny (Plano, TX) Assignee(s): TUFF-LINKS, LLC (Plano, TX) Law Firm: Wick Phillips Gould Martin LLP (Local + 1 other metros) Application No., Date, Speed: 29575247 on 08/23/2016 (840 days app to issue)

“Farmers are losing money,” Mr. Singh said. “We just survive. We are earning less and spending more.”

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